Fig. 4.

Linear fluorescence spectra and corresponding linear quantitative analysis curves. (a) Fluorescence emission spectra and linear relationgship for HIV-1 with different conditions from 0.2 to 700 nM. (b) Fluorescence emission spectra and linear relationgship for HIV-2 with different conditions from 0.2 to 700 nM. I₀ is the fluorescence intensity without the target, and I is the fluorescence intensity after adding the target. The error bar represents the standard deviation of three measurements. PBS buffer (20 mM, pH 7.0); Reaction time of the hybridization of the probe and HIV DNAs: 40 min; Work temperature: 37 °C.