Table 1.
Antiviral activity of Niclosamide and Nitazoxanide against various alphaviruses in vitro.
| Cell line | Virus strains | Niclosamide (μM) |
Nitazoxanide (μM) |
||||
|---|---|---|---|---|---|---|---|
| EC50a | CC50b | SIc | EC50a | CC50b | SIc | ||
| BHK-21 | CHIKV | 0.95 ± 0.22 | >20 | >21.05 | 2.96 ± 0.18 | 25 | 8.45 |
| CHIKV 0611aTw | 0.85 ± 0.12 | >20 | >23.53 | 1.96 ± 0.48 | 25 | 12.76 | |
| CHIKV 0810bTw | 0.90 ± 0.12 | >20 | >22.22 | 4.95 ± 0.23 | 25 | 5.05 | |
| SINV | 1.07 ± 0.40 | >20 | >18.69 | 1.53 ± 1.10 | 25 | 16.34 | |
| SFV | 1.79 ± 0.23 | >20 | >11.17 | 3.56 ± 0.27 | 25 | 7.02 | |
| U2OS | CHIKV | 0.36 ± 0.08 | >20 | >55.55 | 3.01 ± 0.61 | 25 | 8.31 |
a
The EC50s were determined using RT-qPCR and were presented as means ± SD (n ≥ 3).
b
The CC50s were determined using a CCK-8 assay and were presented as means ± SD (n ≥ 3).
c
The SI (selectively index) represented the ratio of CC50 to EC50.