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. 2009 Oct 14;24(2):104–106. doi: 10.1016/j.mcp.2009.10.001

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Copyright © 2009 Elsevier Ltd. All rights reserved.

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Fig. 1 — Primer design of RT-LAMP. Six distinct regions are designated on the target of infectious bronchitis virus. F2c, sequence complementary to F2; F3c, sequence complementary to F3; B2c, sequence complementary to B2; B3c, sequence complementary to B3. Two inner primers (FIP and BIP) and outer primers (F3 and B3) are used in RT-LAMP method. FIP (BIP) is a hybrid primer consisting of the F1c (B1c) sequence, a linker of TTTT and the F2 (B2) sequence.