Table 1.
Ingredients and chemical composition of the experimental diets.
| Item | Treatment1 |
||
|---|---|---|---|
| T0 | T400 | T800 | |
| Ingredients, g/kg (as fed) | |||
| Mannoproteins2 | – | 0.4 | 0.8 |
| Maize, grain | 100 | 99.6 | 99.2 |
| Poultry by-product, meal | 360 | 360 | 360 |
| Ground rice | 400 | 400 | 400 |
| Poultry fat | 80.5 | 80.5 | 80.5 |
| Beet pulp | 20 | 20 | 20 |
| Palatant, liquid3 | 25 | 25 | 25 |
| Potassium chloride | 4 | 4 | 4 |
| Refined salt | 4 | 4 | 4 |
| Vitamin-mineral premix4 | 3 | 3 | 3 |
| Choline chloride | 2 | 2 | 2 |
| Mould inhibitor5 | 1 | 1 | 1 |
| Antioxidant6 | 0.5 | 0.5 | 0.5 |
| Chemical composition | |||
| Dry matter, g/kg (as fed) | 936 | 933 | 941 |
| Crude protein, g/kg (DM) | 319 | 307 | 320 |
| Acid-hydrolized fat, g/kg (DM) | 123 | 124 | 127 |
| Crude fibre, g/kg (DM) | 16 | 17 | 17 |
| Ash, g/kg (DM) | 91 | 83 | 88 |
| Calcium, g/kg (DM) | 17 | 16 | 17 |
| Phosphorus, g/kg (DM) | 11 | 10 | 11 |
T0 – control, with no addition of mannoprotein [six adult (three males and three females) and six elderly dogs (three males and three females)]; T400 – addition of 400 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)]; T800 - addition of 800 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)].
ActigenTM, Alltech, Lexington, KY (CP =280 g/kg; recommended addition: 200–800 g/ton – information provided by the manufacturer).
Liquid palatant enhancer, SPF do Brasil, Descalvado, Brazil.
Provided per kg of diet: Fe, 100 mg as iron sulfate; Cu, 10 mg as copper sulfate; Mg, 10 mg as magnesium oxide; Zn, 150 mg as zinc sulfate; I, 2 mg as potassium iodide; vitamin A (retinol), 18,000 IU; vitamin D (colecalciferol), 1,200 IU; vitamin E (α-tocoferol), 200 IU; thiamine, 6 mg; riboflavin, 10 mg; pantothenic acid, 40 mg; niacin, 60 mg; pyridoxine, 6 mg; folic acid, 0.30 mg; and cobalamin, 0.01 mg.
MoldZap Aquativa, Alltech do Brasil Agroindustrial Ltd., Curitiba, PR, Brazil: ammonium dipropionate, propanediol, propionic acid, acetic acid, lactic acid, ascorbic acid, formic acid, and potassium sorbate.
Banox, Alltech Brasil Agroindustrial Ltd.: BHA, BHT, propyl gallate and calcium carbonate.