Table 4.

Leucocyte subpopulations of adult and elderly dogs before and after consumption of experimental diets on days 1, 14, and 28.

Item	Treatment1						Mean	SEM2	P-value
	Adult			Elderly
	T0	T400	T800	T0	T400	T800			Diet	Age	Time	Diet x Age	Time x Diet	Time x Age	Time x Age x Diet
CD5+, x 103/μL
Day 1	1.32	1.49	1.24	1.27	1.32	1.29	1.32	0.053	0.103	0.013	0.463	0.127	0.524	0.295	0.158
Day 14	1.24	1.32	1.31	0.87	1.26	1.21	1.20
Day 28	1.15	1.99	1.49	1.27	1.17	1.28	1.39
CD4+, x 103/μL
Day 1	0.67	0.67	0.54	0.49	0.49	0.60	0.58a	0.022	0.774	0.345	<0.001	0.608	0.370	0.395	0.164
Day 14	0.39	0.43	0.41	0.23	0.37	0.41	0.37b
Day 28	0.44	0.81	0.60	0.47	0.42	0.46	0.53b
CD8+, x 103/μL
Day 1	0.25	0.28	0.25	0.32	0.31	0.27	0.28a	0.012	0.218	0.779	<0.001	0.472	0.773	0.399	0.865
Day 14	0.08	0.14	0.11	0.09	0.16	0.10	0.11b
Day 28	0.14	0.21	0.18	0.16	0.17	0.16	0.17b
CD21+, x 103/μL
Day 1	0.33	0.31	0.24	0.21	0.22	0.18	0.25a	0.022	0.690	0.013	0.002	0.602	0.892	0.805	0.619
Day 14	0.05	0.07	0.10	0.06	0.04	0.04	0.06b
Day 28	0.13	0.22	0.19	0.16	0.09	0.11	0.15b
CD14+, x 103/μL
Day 1	0.46	0.42	0.52	0.42	0.44	0.44	0.45a	0.021	0.73	0.490	<0.001	0.988	0.650	0.845	0.186
Day 14	0.31	0.23	0.30	0.23	0.18	0.24	0.25b
Day 28	0.37	0.38	0.35	0.33	0.24	0.35	0.34b
CD4+/CD8+
Day 1	2.74	2.59	2.58	1.98	2.12	2.33	2.39a	0.154	0.416	0.002	<0.001	0.284	0.761	0.629	0.191
Day 14	5.48	3.11	4.15	2.95	2.83	4.02	3.76b
Day 28	3.68	4.10	3.80	3.59	2.88	3.09	3.52b

¹T0 – control, with no addition of mannoprotein [six adult (three males and three females) and six elderly dogs (three males and three females)]; T400 – addition of 400 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)]; T800 - addition of 800 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)].

²Standard error of the mean.

^{A, B} - The averages followed by different letters in the columns differ from each other, as determined by Student’s t-test (P ≤ 0.05).