Table 5.

Delayed cutaneous hypersensitivity response of adult and elderly dogs after consumption of experimental diets for 28 days.

Item	Treatment1						Mean	SEM2	P-value
	Adult			Elderly
	T0	T400	T800	T0	T400	T800			Diet	Age	Time	Diet x Age	Time x Diet	Time x Age	Time x Age x Diet
Saline solution, mm
0 Hours	3.43	3.39	3.6	3.3	3.07	3.13	3.32ª	0.055	<0.001	<0.001	<0.001	0.503	0.506	0.691	0.282
24 Hours	2.80	2.58	2.84	2.49	2.46	2.61	2.63b
48 Hours	2.70	2.54	2.73	2.40	2.40	2.55	2.55b
72 Hours	2.78	2.54	2.89	2.56	2.39	2.53	2.62b
Phytohemagglutinin solution, mm
0 Hours	3.39	3.2	3.52	3.04	2.87	3.05	3.18	0.052	0.004	0.148	<0.001	0.908	0.471	0.002	0.759
24 Hours	3.46	3.37	3.77	3.47	3.46	3.78	3.55
48 Hours	3.19	3.06	3.4	3.27	2.97	3.65	3.26
72 Hours	3.07	3.01	3.27	2.96	2.73	2.94	3.00
Vaccine, mm
0 Hours	3.77	3.62	3.96	3.57	3.25	3.19	3.56c	0.061	0.696	<0.001	<0.001	0.002	0.639	0.075	0.633
24 Hours	4.32	4.25	4.50	4.12	4.28	4.12	4.27ª
48 Hours	4.35	4.28	4.43	4.00	4.05	3.97	4.18b
72 Hours	4.23	4.19	4.40	3.80	4.03	3.63	4.05b

¹T0 – control, with no addition of mannoprotein [six adult (three males and three females) and six elderly dogs (three males and three females)]; T400 – addition of 400 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)]; T800 - addition of 800 mg/kg mannoproteins [six adult (three males and three females) and six elderly dogs (three males and three females)].

²Standard error of the mean.

^{A, B} - The averages followed by different letters in the columns differ from each other, as determined by Student’s t-test (P ≤ 0.05).