Fig. 4.

PregSure^®BVD induces alloreactive, surface binding antibodies. (A) Five guinea pigs per group were immunized twice with PregSure^®BVD or three alternative, commercially available BVD vaccines. After 6 weeks sera were analyzed by western blot as described for Fig. 2D. Five sera per group were tested and the strips are grouped accordingly. (B) The same sera were tested by flow cytometry for the presence of antibodies binding to the cell surface of bovine leukocytes. Results are shown for one representative experiment. Black dots represent the MFI of PHA blasts from a 1-year-old heifer after staining with individual immune sera, black bars indicate the median value for each group. P-Values were calculated by simple Student's t-test. (B) Two calves were vaccinated four times with PregSure^®BVD. At the indicated time points serum was obtained and tested for alloreactive binding to the bovine kidney cell line used for vaccine production (upper panel) or PHA blasts from a 1-year-old heifer (lower panel). Open symbols represent the MFI for one calf 1, filled symbols represent the other calf 2.