Fig. 2.

RT-PCR analysis of liver mRNA using specific primers for murine MT-I and β-actin under condition described in Section 2 in young transgenic mice overexpression MT and respective controls after partial hepatectomy/liver regeneration. [(□), sham; (■), at 48 h; (▧), 15th day]. After mRNA isolation and cDNA synthesis, the amount of mRNA for MT-I was determined by semi-quantitative PCR. The densitometry analysis was also performed using Gel-Doc instrument (Bio-Rad). The results are shown in the histograms and are expressed as MT-I/β-actin ratio. No differences exist in immune parameters between Balb/c and C5BL/6J mice used as controls of MT-I* mice (Mocchegiani et al., 2002a).