Fig. 3.

Liver c-myc mRNA (RT-PCR) concentrations in young, old, very old and MT-I* mice after partial hepatectomy/liver regeneration. RT-PCR analysis was performed using specific primers for mouse c-myc and β-actin under conditions described in Section 2. After mRNA isolation and cDNA synthesis, the amount of mRNA for c-myc was determined by semi-quantitative PCR. The densitometry analysis was also performed using Gel-Doc instrument (Bio-Rad). The results are expressed as c-myc/β-actin ratio. *P<0.01 as compared with young sham (time 0) and to young pHx mice (15th day), respectively.