Fig. 7.

Predicted structure of GP5 and M heterodimer. The predicted N-glycosylation sites (Asn-56 and Asn-81) are depicted with an orange circle. The green circle represents the N-glycosylation site (Asn-73) found in isolates of an extensive recent EVA outbreak in North America. The GP5 and M proteins are covalently linked by a disulfide bond (S—S) formed between Cys-34 in the GP5 protein and Cys-8 in the M protein. The major neutralization determinants of EAV that are located in the N-terminal ectodomain of the GP5 major envelope glycoprotein of EAV are depicted. The signal sequence (aa 1–18), four major neutralization sites (A, B, C and D) and putative glycosylation sites (aa positions 56, 73 and 81) are identified.