Fig. 10.

Dual-color immunofluorescence flow cytometry analysis of T cells from carrier and seropositive non-carrier stallions following in vitro infection with EAV. (A) Carrier status was confirmed by repeatedly isolating EAV from semen over an extended period of time. (B) Non-carrier stallions were uninfected but seropositive to EAV following natural infection, not vaccination. Lymphocytes from each stallion that were labeled with both anti-CD3 and anti-nsp1 (12A4) MAbs are shown in the upper right quadrant of each dot plot. Note significant double labeling of lymphocytes from the carrier stallion but not the non-carrier stallion for both CD3 and EAV after in vitro infection.

Modified from Go et al. (2012) with permission from the Journal of Virology.