Table 1.

Nonstructural and structural proteins of EAV.^a

ORF	Protein	Nucleotide position (bp)	Protein length (aa)	Predicted M.W. (kDa)
ORF1a	1a polyprotein	225–5399	1725	190
ORF1b	–	5405–9751	–	–
ORF1ab	1ab polyprotein	225–9751	3176	349.3
	nsp1	255–1004	260	28.6
	nsp2	1005–2717	571	61.4
	nsp3	2718–3416	233	25
	nsp4	3417–4028	204	21
	nsp5	4029–4514	162	18.1
	nsp6	4515–4580	22	2.3
	nsp7	4581–5255	225	25.2
	nsp8	5256–5405	50	5.5
	nsp9	5256–7333	693	76.8
	nsp10	7334–8734	467	50.5
	nsp11	8735–9391	219	24.2
	nsp12	9392–9748	119	12.5
ORF2a	E	9751–9954	67	7.4
ORF2b	GP2	9824–10,507	227	25.0
ORF3	GP3	10,306–10,797	163	17.9
ORF4	GP4	10,700–11,158	142	15.6
ORF5a	ORF5a	11,112–11,291	59	6.5
ORF5b	GP5	11,146–11,913	255	28.0
ORF6	M	11,901–12,389	162	17.8
ORF7	N	12,313–12,645	110	12.1

^aBased on EAV VBS (ATCC VR-796) published sequence (GenBank accession number DQ846750).