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. 2001 Jun 26;88(3):183–204. doi: 10.1016/S0168-1656(01)00281-4

Fig. 3.

Fig. 3

Summary of helper dependent expression systems based on coronavirus derived minigenomes: A–C. Expression modules based on MHV minigenomes DIssF and DIssE cloned under the control of T7 bacteriophage polymerase (T7), used to express chloramphenicol acetyltransferase (CAT), hemagglutinin-esterase (HE) or interferon-γ using either an IRES (A), or TRSs (B–C). D–E. Expression modules based on the TGEV derived minigenome M39 used to express the GUS. The minigenome was cloned either after T7 (D) or the CMV (E) promoters. F. Expression module based on the IBV derived minigenome CD-61 used to express CAT.