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. 2009 Apr 10;138(3):258–265. doi: 10.1016/j.vetmic.2009.04.006

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Copyright © 2009 Elsevier B.V. All rights reserved.

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Fig. 4 — Microscopic examination of nuclear staining with DAPI. Fluorescence microscopic examination indicated that nuclei of infected cells appeared fragmented (denoted by arrowhead in A, 400×) and marginated (denoted by arrowheads in B, 600×). Typical apoptotic bodies could also be detected (denoted by arrowheads in C and D, 600×). Nuclei of mock-infected cells were uniformly stained without condensation (E, 400×). Multinucleate cell (syncytium) formation (denoted by arrowhead in F, 400×) could also be observed by this method.