Table 1.
RT-LAMP assay primers used for amplification of DENV.
| Primer name | Type | Length | Sequence |
|---|---|---|---|
| FIP DENV1/3 | Forward inner | 49 | GGTTATTCATCAGAGATCTGCTCTCTTTTTTTCGAATCGGAAGCTTCGT |
| FIP DENV2 | Forward inner | 49 | GGTTATTCATCAGAGATCTGCTCTCTTTTATTCTTTGAGGGAGCTAAGC |
| FIP DENV4 | Forward inner | 49 | TTCATTTTTCCAGAGATCTGCTCTCTTTTTTTCGAATCGGAAGCTTCGT |
| BIP DENV1/3 | Reverse inner | 49 | AACGGAAAAAGACGGGTCAACCGTTTTTCTTTGTCAGCTGTTGCACAGT |
| BIP DENV2 | Reverse inner | 49 | AACGGAAAAAGGCGAGAAATACGCTTTTCTTTGTCAGCTGTTGCACAGT |
| BIP DENV4 | Reverse inner | 49 | AACGAAAAAAGGTGGTTAGACCACTTTTCTTCACCAACCCTTGAGGGGT |
| F3 | Forward outer | 18 | GTGGACCGACAAAGACAG |
| B3 DENV1/3 | Reverse outer | 18 | GTGAGCAATCCTTTTGAG |
| B3 DENV2 | Reverse outer | 18 | TGCAGCATTCCAAGTGAG |
| B3 DENV4 | Reverse outer | 18 | GAAAAAAGTCCGGTTGAG |
| LoopB | Reverse loop | 19 | GCGAGAGAAACCGCGTGTC |
| FAM-LoopB | Reverse loop | 20 | [Fam]a-CGCGAGAGAAACCGCGTGTC |
| Antisense LoopB | Reverse loop | 20 | GACACGCGGTTTCTCTCGCG-[Bhq] b |
Final sequences for the optimized primer combination are given. The forward inner primers and backward inner primers contain a TTTT spacer as suggested in previous literature.
a
Oligo labeled with FAM (5 carboxy fluorescein).
b
Oligo labeled with BHQ.