Fig. 2.

Elucidation of a single replicative cycle of Japanese encephalitis virus in porcine stable kidney (PS) cells. PS cells were infected with a multiplicity of infection (MoI) of 1 of JEV at the 0 h time point. Virus yield in the supernatant and JEV antigen in the cells were examined at 2-h intervals up to 24 h post infection. (A) Yield of virus in the supernatant fluid. The x-axis represents the various time points at which virus yield was evaluated and the y-axis represents the virus yield (log 50% tissue culture infective dose (TCID₅₀)/mL). (B) Detection of JEV-specific antigen using an immunofluorescence assay. It can be observed that the earliest appearance of cell-bound antigen was at 10 h post infection, whilst the earlier time points were negative for viral antigen (400×).