Fig. 4.

Western blot illustrating the effect of pentoxifylline on Japanese encephalitis virus translation using an in vitro translation kit. Lane 1, uninfected cell control; lanes 2 and 4, in vitro translation products of RNA obtained from JEV-infected porcine stable kidney (PS) cells (untreated) at 4 and 10 h post infection, respectively; lanes 3 and 5, in vitro translation products of RNA obtained from JEV-infected PS cells treated with pentoxifylline for 4 and 10 h, respectively. Note the presence of a 50-kDa JEV-specific protein in pentoxifylline-treated JEV-infected cells at 4 and 10 h. Lane 6, molecular weight markers.