Table 1.
Summary of immunohistochemical methodology
| Specificity | Type of antibody | Source | Commercial origin | Fixative | Dilution | Antigen retrieval |
|---|---|---|---|---|---|---|
| PRRSV (clone SDOW-17/SR-30) | mAb | Mouse myeloma cells | Rural Technologies Inc. | Bouin's | 1 in 1,000 | HTAR |
| Human macrophages (clone MAC387) | mAb | NS1 Mouse myeloma cell line | Chemicon Europe, Hampshire, UK | Formaldehyde 10% | 1 in 750 | Protease |
| Human IL-1α | pAb | Rabbit serum | Endogen, Woburn, Massachusetts | Bouin's | 1 in 100 | Tween |
| Porcine IL-6 | pAb | Rabbit serum | Endogen | Bouin's | 1 in 10 | Tween |
| Human TNF-α (clone 68B6A3) | mAb | NSO Mouse myeloma cell line | Biosource, Camarillo, California | Bouin's | 1 in 25 | Tween |
| Porcine IFN-α (clone F17) | mAb | Mouse myeloma cells | Prof. K. Van Reeth, University of Ghent, Belgium | Bouin's | 1 in 300 | Tween |
| Porcine IFN-γ | pAb | Goat serum | RnD Systems, Minneapolis, Minnesota | Bouin's | 1 in 20 | Tween |
| Porcine IL-10 | pAb | Goat serum | RnD Systems | Bouin's | 1 in 20 | Tween |
| Porcine IL-12 | pAb | Goat serum | RnD Systems | Bouin's | 1 in 20 | Tween |
mAb, monoclonal antibody; pAb, polyclonal antibody; HTAR, high temperature antigen retrieval with citrate buffer pH 6.0; Protease, protease digestion for 10 min; Tween, incubation in Tween 20 diluted 0.01% in phosphate buffered saline for 10 min.