Table 1.
List of primers and siRNA target sequences used
| Name | Primer sequence | Location | Length |
|---|---|---|---|
| AHV-UL-6B-F | 5′-ATAATCAGGGTCAAGAGGTAGCACT-3′ | 708–1032 | 325 |
| AHV-UL-6B-R | 5′-GGTTCATATCCATCTTCCGCAAATC-3′ | 708–1032 | 325 |
| T7 promoter appended UL-6 primersa | 5′-TAATACGACTCACTATAGGGAGAATAATCAGGGTCAAGAGGTAGCACT-3′ | ||
| 5′-TAATACGACTCACTATAGGGAGAGGTTCATATCCATCTTCCGCAAATC-3′ | |||
| AHV-UL6-AsiRNA | 5′-UUUAGCAAAGGCGCGAAUGUU-3′ | 395 | |
| 3′-UUAAAUCGUUUCCGCGCUUAC-5′ | |||
| AHV-UL6-BsiRNA | 5′-UAACUCUCCUCGUUGCACCUU-3′ | 751 | |
| 3′-UUAUUGAGAGGAGCAACGUGG-5′ | |||
a
For the T7 promoter appended UL-6 primers, promoter sequences are marked in italics, the purine sequences highlighted and the primer sequences underlined.