Fig. 3.

Differences in nucleocapid disorder of various Nipah virus strains. A. PONDR® VLXT plots of the nucleocapsid proteins of various NiV strains listed in Table 1. B. Zoomed-in PONDR® VLXT plots of the C-terminal regions of the nucleocapsid proteins of various NiV strains. C. Disorder “difference spectra” representing differences between the various NiV strains in relation to the Malaysia-Singapore 1998-9 strain. D. Zoomed-in disorder “difference spectra” representing differences between the various NiV strains in relation to the Malaysia-Singapore 1998-9 strain with the focus on the C-terminal regions of the nucleocapsid proteins. In these plots, the corresponding disorder “difference spectra” were calculated by subtracting the PONDR® VLXT profile of the Malaysia-Singapore 1998-9 strain from the PONDR® VLXT profiles of other NiV strain. Positive peaks here correspond to the region of newer strains that are more disordered than the corresponding regions in the Malaysia-Singapore 1998-9 strain, whereas negative peaks show regions with higher disorder in the Malaysia-Singapore 1998-9 strain relative to the newer NiV strains. E. Multiple sequence alignments of N proteins from different NiV strains listed in Table 1. Alignment was conducted by Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) using the default parameters. Residues, which are predicted to be more disordered in the Malaysia-Singapore 1998-9 strain relative to the newer NiV strains are shown by bold blue font, whereas bold red font is used to show residues predicted to be more disordered in the more recent NiV strains relative to the Malaysia-Singapore 1998-9 strain.