Table 4.

Analysis of adsorbed and penetrated fluorescence-labelled IAV by FACS. A549 and MDCK cells were treated or not with 1 and 2.5 mM GL, respectively (treatment protocol F), and infected with fluorescence-labelled IAV at different MOIs. Percentages of gated green fluorescent cells are shown. The results have been reproduced independently at least 3 times.

MOI	% Green cells
	Controla	0.05	0.1	1	2.5	5	10
A549
Adsorption
1 mM GL	0.0	0.27	0.35	5.28	12.52	24.82	52.32
0 mM GL		0.25	0.45	6.60	9.24	28.80	50.98
Uptake
1 mM GL	0.0	0.10	0.25	4.54	7.83	16.44	35.19
0 mM GL		0.33	0.45	8.37	13.99	33.75	60.17
MDCK
Adsorption
2.5 mM GL	0.0	0.2	0.2	5.5	24.1	51.4	55.4
0 mM GL		0.2	0.6	9.2	34.4	60.9	74.4
Uptake
2.5 mM GL	0.0	0.02	0.03	0.09	0.27	1.07	2.49
0 mM GL		0.10	0.12	0.86	1.26	4.24	52.04

^aCells infected with unlabelled IAV virus at MOI 1.