Fig. 1.

Sequence cloning, analysis and three-dimensional prediction of the BoIFN-ω3. (A) PCR identification of mature BoIFN-ω3 gene. Lane M: Trans 2 K DNA marker; Lane 1: mature BoIFN-ω3 gene. (B) The sequence underlined with a wavy line is the signal sequence, while the arrow denotes the signal sequence cleavage site. Cysteine residues forming disulfide bonds are marked with gray circles. The conserved Arg residues (at position 161 of mature BoIFN-ω3) are marked with a gray box. Letters A–E in red refer to α-helices in BoIFN-ω3. (C) Predicted three-dimensional structures of BoIFN-ω3. The five α-helices were labeled A–E.