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(A) pH and (B) temperature sensitivities of BoIFN-ω3. The purified BoIFN-ω3 was adjusted to pH 2.0, 42 °C, 56 °C, 63 °C and then finally reverted to the original pH (7.0) or cooled down in an icebox. Afterwards, antiviral activities were separately analyzed in MDBK-VSV system. After acid and high temperature treatment, BoIFN-ω3 retained its antiviral activity with no significant difference from that of the control group.
