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. 2017 Feb 10;115:125–131. doi: 10.1016/j.rvsc.2017.01.028

Fig. 6.

Fig. 6

Analysis of ISGs transcription and Mx-1 protein expression in MDBK cells after BoIFN-ω3 treatment. (A) Transcription analysis of ISGs in MDBK cells after BoIFN-ω3 treatment. Quantitative real-time RT-PCR was used to detect the changes in Mx1, ISG15 and ISG56 transcription levels. MDBK cells without BoIFN-ω3 treatment were used as control, while GAPDH was used as the internal reference. Data are presented in mean ± SD (*, P ≤ 0.05; **, P ≤ 0.01). (B) Expression analysis of Mx-1 protein in MDBK cells after BoIFN-ω3 treatment. MDBK cells without BoIFN-ω3 treatment were used as control, while GAPDH was used as the internal reference. Lane M: EasySee Western marker; Lane 1: Control cells; and Lane 2–6: cell lysates collected at 3, 6, 9, 12, and 24 h after treatment with BoIFN-ω3.