Fig. 2.

Vaccination induced IBV specific IgA concentration in the tear of SPF chicken in the presence or absence of R-848. Birds of 2 week age were immunized with live or inactivated IBV vaccine in the presence or absence of R-848 (50μg/bird) with a booster 14 days later. IgA response was monitored after booster at weekly interval till 35 dpi (n = 6/group on dpi 21, 28 and n = 3/group on 35 dpi). The experiment was repeated twice independently and data from the first experiment was used for analysis. Treatment effect was analyzed at each time point by One way ANOVA with Duncan's multiple range test to compare the pair-wise mean difference. Alpha error was set at 1%. **Indicate significant difference (P < 0.01) between the groups. dpi: day post-immunization.