Fig. 1.

Sequence characteristics of LsACE. (A) The amino acid sequence of LsACE. The secretory signal peptide is underlined. N-glycosylation sites are marked with triangles. Active site sequences are boxed. The transmembrane region at the C-terminal is underlined with two lines. (B) The phylogenetic tree of LsACE and six homologs of Drosophila melanogaster constructed with the neighbor-joining method using pairwise deletion and the p-distance model. Bootstrap analysis with 1000 replicates was performed. The accession numbers for D. melanogaster ACE homologs are NP_477195.1 for DmACER, NP_477046.1 for DmAnCE, NP_788042.1 for DmAnCE2, NP_001033904.1 for DmAnCE3, NP_610442.2 for DmAnCE4, and NP_573392.2 for DmAnCE5.