Figure 5.

Generation of LSECtin KO mice. (A) The targeting map of the LSECtin genomic locus. The whole coding region was replaced with PGK-neo cassette. RE sites: N, Nco I; Bg, Bgl II; RV, EcoR V. The short black bar indicates the external southern probe, and short arrows are genotyping primers. (B and C) Southern blot and PCR detection of heterozygous and homozygous LSECtin mutants in genomic DNA from targeted (B) embryonic stem (es) cells and (C) mouse tails. (D and E) The expression of LSECtin in liver from wt or KO mice estimated by (D) reverse-transcription PCR analysis and (E) immunofluorescein staining.