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Architecture of Mediator head. The seven-subunit, 223-kDa complex was produced by MultiBac, crystallized and the X-ray structure determined [35]. (a) A representative gel section from SDS-PAGE showing the quality of sample prepared. (b) The crystal structure in a schematic view (top) and in an identically color-coded ribbon representation superimposed on the experimental electron density (bottom). Density is displayed at 4 Å resolution (contoured at 1.5 σ).
