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. 2013 Dec 11;171:17–24. doi: 10.1016/j.jbiotec.2013.11.022

Fig. 1.

Fig. 1

Generation of transgenic pigs expressing siRNA. The plasmids were linearized and transfected into pig fibroblast cells. Cell clones containing the siRNA-expressing cassette were isolated by G418 screening and served as donors for SCNT. Finally, pigs that endogenously expressed the siRNA were generated via SCNT.