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. 2013 Dec 11;171:17–24. doi: 10.1016/j.jbiotec.2013.11.022

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Copyright © 2013 Published by Elsevier B.V.

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Fig. 6 — Transgenic siRNA in PAMs inhibited PRRSV replication. (a) qRT-PCR analysis of siRNA expression in PAMs isolated from TG pigs. The expression of siRNA was expressed as a relative ratio to U6. Data are presented as mean ± SD from three TG pigs. (b) qRT-PCR analysis of viral ORF7 RNA from TG and NTG PAMs both inoculated with PRRSV JXA1 for 12, 24 and 48 h. Fold change was generated by 2^−ΔΔCt. ΔCt = Ct(viral RNA) − Ct(GAPDH); ΔΔCt = ΔCt − Ct (reference, viral RNA at pi 12 h). The default fold of viral RNA at pi 12 h was 1. Data are representative of the results of three individuals (means ± SD). (c) PAMs of TG and NTG pigs were observed for development of CPE by bright-field microscopy at 24 h pi. (d) Immunofluorescence assay analysis of PRRSV N protein in TG and NTG PAMs infected with PRRSV JXA1 for 12 and 24 h.