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Determination of antigen-specific induction of the frequency of IFN-γ producing PBMC in ELISPOT assays (2 × 105 cells/well, 72 h) either re-stimulated with PRV (C) or stimulated with the closely related alpha herpesvirus BHV-1 (B). Only slightly elevated background spot numbers compared to culture medium controls (A) are present when BHV-1 is used as the stimulating virus in PRV primed PBMC from PRV challenge surviving piglets.
