Table 1.
Evaluation of rapid MERS-CoV assay.
| Evaluation | Rapid MERS-CoV assay results |
|---|---|
| Initial evaluation [Concentration of spiked MERS-CoV (log10 TCID50/ml)] | |
| 5.2 | Positive |
| 4.2 | Positive |
| 3.7 | Positive |
| 3.2 | Negative |
| 2.2 | Negative |
| Evaluation using human NPAs | |
| NPAs positive for various respiratory viruses (n = 56)a | 100% negative |
| NPAs spiked with MERS-CoV (n = 10) | 100% positive |
| Evaluation using dromedary nasal swabs/tonsils | |
| RT-PCR positive (n = 16) | 81% positive |
| RT-PCR negative (n = 65) | 100% negative |
a
Include influenza A virus (n = 5), influenza B virus (n = 5), influenza C virus (n = 2), parainfluenza virus 1 (n = 5), parainfluenza virus 2 (n = 5), parainfluenza virus 3 (n = 5), parainfluenza virus 4 (n = 5), human rhinoviruses (n = 5), human metapneumovirus (n = 5), human adenovirus (n = 2), respiratory syncytial virus (n = 2), human bocavirus (n = 2), human CoV HKU1 (HCoV-HKU1) (n = 2), HCoV-OC43 (n = 2), HCoV-229E (n = 2) and HCoV-NL63 (n = 2).