Table 1.
Sequences of primers used.
| Name | Sequence (5′to 3′) | Application |
|---|---|---|
| F1 | TTCTGGAACGCTCTTCTCACA | promoter amplification |
| R1 | GCACCTTGTCCTTCTCCTCT | |
| PciI/BamHIF | GCTCACATGTTTCTGGAACGCTCTTCTCACA | plasmid construction |
| PciI/BamHI R | GGTGGATCCCGCTGCGCTGGCGGCGCCCGGGCCGGCTCTGCTGCT | |
| SfiI/NheI F | GGGGAAAAGGCCTCCAAGGCCTTCTGGAACGCTCTTCTCACA | plasmid construction |
| SfiI/NheI R | ATTCGCTAGCCTTAAGATACATTGATGAGTTTGG | |
| DsRed F | AGTTCCAGTACGGCTCCAAGGT | RT-PCR |
| DsRed R | CAGCCCATGGTCTTCTTCTGCATT | |
| IFN-α F | AACGCCAAAGCCTCCTCAAC | RT-PCR |
| IFN-α R | TTGTCTTGGAGGAAGGTGTG | |
| IFN-β F | TCCTGCAACCATCTTCGTCA | RT-PCR |
| IFN-β R | GCTGCTTGCTTCTTGTCCTT | |
| MDA5 F | CTGGTTGGGATTGAGACTGT | RT-PCR |
| MDA5 R | GTCTTTATCCTCTGTTCCTCCTG | |
| β-actin F | TACGTCGCACTGGATTTCGA | RT-PCR |
| β-actin R | CAGGACTCCATACCCAAGAA |