Table 1.

Primers for the feline genes of interest (GOI). For the sequences of the used reference genes, see Penning et al. (2007).

Gene	Acc	Forward oligo (5′ → 3′)	Reverse oligo (5′ → 3′)	Tm (°C)	Fragment (bp)
IL-1β	NM_001009351	ATGACCCACTTCATGAGGACTG	TCACCACACTCTTCTTGAGGG	64	99
IL-6	NM_001009211	GACTTCCTTCAGTTCAGCCTCAGG	AGGAATGCCCGTGAACTACAGC	61	81
IL-10	NM_001009209	ACAGCACGTGAACTCCCTGG	TCTTCACCTGCTCCACCACC	66	113
TNF-α	NM_001009835	TCTTCTCCTTCCTCCTCGTCG	GGGGTTTGCTACTACATGGGC	65	185
IFN-γ	NM_001009873	CAGATGTAGCAGATGGTGGGTC	CATGTCTTCCTTGATGGTGTCC	60	176
nVDR	XM_543714	TCTCTGACCCTGGACCTGTC	GAAGTGAGGTCTCTGAACCCTG	62	122
RANKL	DQ027064	AGCCTGATACCCAGCCT	GATACTCTGTGGCGAGGTC	58	230
OPG	XM_539146	GGGTTCTTCTCGAATGAGACG	CCTGAAGAATGCCTCCTCAC	59	183