Table 2.
Primers and probes used in this study.
| Gene | Name | Sequence (5′-3′)a | Accession no. | Reference |
|---|---|---|---|---|
| matrix | AMPB | GATCACtaatacgactcactatagggCAGAGACTTGAAGATGTCTT | NC_026431 | [15] |
| AMPC | TGCTGGGAGTCAGCAATCTG | |||
| MF146 | GGCTCTCATGGAATGGCTAAAGACAAGAC | This study | ||
| MR425 | Biotin-TTGTATATGAGGCCCATGCAACTGGCAAGTG | |||
| A-p | FITC-TTCACGCTCACCGTGCCCAGTGAGCGAGGAC-THF-GCAGCGTAGACGCTTTG-spacer(C3) | |||
| H1 | H1F1073 | GATCACtaatacgactcactatagggGGTAGATGGATGGTACGGTT | CY225830 | This study |
| H1F1325 | Biotin-TGTTGGTTCTATTGGAAAATGAAAGAACTTT | |||
| H1-P | FITC-TCATAAGTCCCATTTTTGACACTTTCCATGC-THF-CGTGTTATCGCATTTG-spacer(C3) | |||
| H1R1540 | TGTTTAATTTTGCTTCCTCTGAGTATTTTGG | |||
| H3 | H3F376 | GATCACtaatacgactcactatagggTTATGCCTCCCTTAGGTCAC | CY225422 | This study |
| H3F763 | Biotin-GAATAAGCATCTATTGGACAATAGTAAAAC | |||
| H3-P | FITC-ATGGGTGCATCTGATCTCATTATTGAGCTTT-THF-CCCACTTCGTATTTTG-spacer(C3) | |||
| H3R1156 | CCCTCAGAATTTTGATGCCTGAAACCGTACCA |
a
Protecting bases are shown in boldface, and the T7 promoter at the 5′ end of forward primer is shown in lowercase.