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Probit regression to calculate the detection limits of RT-RPA-LFD assays.
| Amount of RNA transcripts (Copies/reaction) | Replicates detected/Replicates tested by RT-RPA-LFD assays |
||
|---|---|---|---|
| matrix | H1 | H3 | |
| 5000 | 8/8 | 8/8 | 8/8 |
| 500 | 8/8 | 7/8 | 8/8 |
| 100 | 7/8 | 2/8 | 7/8 |
| 50 | 5/8 | 0/8 | 6/8 |
| 10 | 0/8 | 0/8 | 0/8 |
| LOD (95% probability) | 123.6 | 677.1 | 112.2 |
