Table 1.

Oligonucleotide primer sequences used.

	Name	Primers (5′… 3′)	Function
1	GFP ins+	GGGACCTCGAGTATGGTGAGCAAGGGCGAGGAGC	GFP amplification adding XhoI sites
2	GFP ins neg	CCACTCCTCGAGATTTTACTTGTACAGCTCGTCC	GFP amplification adding XhoI sites
3	N all b neg	ACTAATGAGAATCACAATAATAAAAAGCACAG	N RT and PCR amplification
4	N 200+	GCAGCATGGATACTGGAGACG	N sequencing
5	N 300 neg	GGTCAGCGGCTGGTCCTGTTCC	N sequencing
6	N 560+	GGTTCACGTGGTCGTAGGAG	N sequencing
7	N 750+	CCAGGTTATAGAGTAGATCAAGTATTTGGC	N sequencing
8	N 920+	CTGTGGTGCCTAGAGATGACC	N primer for mRNA PCR
9	N all+	CCAAGGGAAAACTTGTGAGGAACAC	N PCR amplification
10	N start xho+	GGAACACTATTATAATAACAATCCTCGAGCATGGCAAGCAGTAAGG	N amplification adding sticky ends
11	N stop xho neg	TGTAGCAAGTCCTTACTCGAGTCAAAGTTCATTTTCACCAAG	N amplification adding sticky ends
12	QX 1210 neg	ACATTCAAAATTCATGCTTAA	Diagnostic RT-PCR for QX IBV
13	QX 860+	TGTTAATACTACTCTGGCG	Diagnostic RT-PCR for QX IBV
14	QX S1 1050+	GGTTTAATTCCTTGTCAGTTTCTCTTACTTATGG	S1 sequencing
15	QX S1 1380+	GCTGCTAATTTTAGTTATTTAGCAGATGGTGG	S1 sequencing
16	QX S1 270 neg	CCTGAAGAGGTGCTGTCATAGC	S1 sequencing
17	QX S1 400+	GGCATGATTCCACGTGATCATATTCG	S1 sequencing
18	QX S1 550 neg	CAGTAGTTTTGTTGGAAGTAAAAACAAGATCACC	S1 sequencing
19	QX S1 end neg	CGAACCATCTGGTTCAATACAAAATCTGC	S1 PCR amplification
20	QX S1 start+	CCAGTTGTGAATTTGAAGAAAGAACAAAAGACCGACTTAG	S1 PCR amplification
21	RT QX S1 neg	CATCTTTAACGAACCATCTGG	S1 RT amplification
22	S1 1380+	GCTGCTAATTTTAGTTATTTAGCAGATGGTGG	S1 primer for mRNA PCR
23	S1 start xho+	GGTAAATTATTGCTCGAGGATGTTGGTGAAGTCACTGTTTTTAGTG	S1 amplification adding XhoI sites
24	S1 stop xho neg	GTTACGTTTTGCTCGAGTTAACGCCTACGACGATGTGAGCTATTGG	S1 amplification adding XhoI sites
25	SX 3+	TAATACTGGYAATTTTTCAGA	S1 sequencing