Table 1.

Anti-HCMV activity of the synthesized compounds in HEL cell cultures.

Compd	R1	R2	n	mp (°C)	Yield (%)	EC50 (μМ)a		Cytotoxicity (μМ)
						AD-169	Davis	Cell morphology (MCC)b	Cell growth (CC50)c
9	H	H	3	151–153	88	>100	>100	>100	>100
10	H	2-Me	3	147–149	72	>100	>100	>100	>100
11	H	3-Me	3	101–103	75	45.0	55.0	>100	>100
12	H	4-Me	3	146–147	87	>20	>20	100	>100
13	H	3,5-Me2	3	96–98	85	>100	>100	>100	>100
14	H	4-iPr	3	139–140	78	25.0	15.0	100	>100
15	H	4-tBu	3	125–127	77	14.0	12.0	100	40
16	H	4-Ph	3	174–175	76	>20	54	100	ND
17	H	4-CN	3	185–186	84	8.9	5.5	100	100
18	H	4-CO2nPr	3	131–132	76	45.0	45.0	⩾100	>100
19	H	4-Cl	3	168–169	84	>100	>100	>100	ND
20	H	4-Br	3	125–127	76	9.4	12.0	100	>100
21	H	4-NO2	3	197–199	78	>4	>4	20	ND
22	Br	4-Br	3	160–162	77	36.0	67.0	>100	>100
23	I	4-Br	3	166–168	78	13.0	20.0	⩾100	>100
24	Me	4-Br	3	169–171	82	8.9	9.0	100	>100
25	H	4-Br	1	179–181	79	>100	>100	>100	ND
26	H	4-Br	2	143–144	80	>20	>20	100	ND
27	H	4-Br	4	121–122	70	>4	>4	20	ND
28	H	4-Br	6	145–147	76	12.0	8.9	⩾100	>100
29	H	4-CO2H	3	225–227	81	>100	>100	>100	ND
30	H	4-NH2	3	190–192	59	55.0	55.0	>100	>100
Ganciclovir						7.0	8.3	394	200
Cidofovir						1.3	1.1	317	161

^aEffective concentration required to reduce virus plaque formation by 50%. Virus input was 100 plaque forming units (PFU).

^bMinimum cytotoxic concentration that causes a microscopically detectable alteration of cell morphology.

^cCytotoxic concentration required to reduce cell growth by 50%.