Table 2.
RT-LAMP for detecting clinical specimens from HFMD patients and a comparison with RT-PCR
| Clinical patients identified with HFMD | Total number of detected specimens | Detection methods |
|||
|---|---|---|---|---|---|
| RT-LAMPa |
RT-PCR |
||||
| Positive | Negative | Positive | Negative | ||
| EV71 | 100 | 97 | 3 | 83 | 17 |
| EV71/CA16 double infected | 4 | 4 | 0 | 4 | 0 |
| CA16 | 4 | 0 | 4 | 0 | 4 |
a
In comparison with the RT-PCR assay, the sensitivity of RT-LAMP was 97.1% (101/104), whereas RT-PCR exhibited a sensitivity of 83.7% (87/104); both methods had a specificity of 100%.