Table 3.
Effect of cryopreservation on the detection ability of RT-LAMP and RT-PCR assay
| Specimen groups | Samples collected duration (number of freezing and thawing times)a | Detection methods |
|||
|---|---|---|---|---|---|
| RT-LAMP |
RT-PCR |
||||
| Positive | Negative | Positive | Negative | ||
| 1 | 2009.8–2009.10 (0–2) | 97.1% (101/104)b | 2.9% (3/104) | 83.7% (87/104) | 16.3% (17/104) |
| 2 | 2009.6–2009.7 (3–5) | 90% (36/40) | 10% (4/40) | 65% (26/40) | 35% (14/40) |
| 3 | 2009.3–2009.5 (>5) | 58% (29/50)b | 42% (21/50) | 54% (27/50) | 46% (23/50) |
a
Freezing and thawing mean that the samples after being collected from patients were stored at −70 °C and thawed to room temperature before use.
b
Number of positive or negative specimens/total detected specimens in this group.