Table 1.
Viral detection by in house singleplex CDC and commercial FTD® Respiratory Pathogens 33 in 217 respiratory specimens collected in Cameroon.
| Targets | FTD-33 |
Singleplex CDC |
FTD-33/singleplex CDC |
PPA | NPA | kappa [CI 95%] | P-value⁎ | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| + | - | + | - | +/+ | −/− | +/− | −/+ | |||||
| RSV | 74 (34.1) | 143 (65.9) | 73 (33.5) | 144 (66.5) | 72 | 142 | 2 | 1 | 97.3 | 99.3 | 0.97 [0.92–1] | 1 |
| HMPV | 5 (2.3) | 212 (97.7) | 7 (3.2) | 210 (96.8) | 5 | 210 | 0 | 2 | 100 | 99.1 | 0.83 [0.49–1] | 0.5 |
| PIV 1 | 10 (4.6) | 207 (95.4) | 13 (6) | 204 (94) | 9 | 203 | 1 | 4 | 90 | 98.1 | 0.77 [0.53–0.94] | 0.3 |
| PIV 2 | 6 (2.8) | 211 (97.2) | 7 (3.2) | 210 (96.8) | 6 | 210 | 0 | 1 | 100 | 99.5 | 0.92 [0.66–1] | 1 |
| PIV 3 | 17 (7.8) | 200 (92.2) | 17 (7.8) | 200 (92.2) | 13 | 196 | 4 | 4 | 76.5 | 98 | 0.75 [0.53–0.9] | 1 |
| PIV 4 | 4 (1.8) | 213 (98.2) | 4 (1.8) | 213 (98.2) | 1 | 210 | 3 | 3 | 25 | 98.6 | 0.24 [−0.02–0.66] | 1 |
| AdV | 78 (35.9) | 139 (64.1) | 85 (39.2) | 132 (60.8) | 72 | 126 | 6 | 13 | 92.3 | 90.6 | 0.81 [0.73–0.88] | 0.1 |
| RV | 58 (26.7) | 159 (73.3) | 55 (25.3) | 162 (74.7) | 50 | 154 | 8 | 5 | 86.2 | 96.9 | 0.84 [0.75–0.92] | 0.5 |
| HCoV-229E | 2 (0.9) | 215 (99.1) | 2 (0.9) | 215 (99.1) | 2 | 215 | 0 | 0 | 100 | 100 | 1 [1–1] | 1 |
| HCoV-OC43 | 7 (3.2) | 210 (96.8) | 13 (6) | 204 (94) | 7 | 204 | 0 | 6 | 100 | 97.1 | 0.69 [0.38–0.89] | 0.03 |
| HCoV-NL63 | 1 (0.5) | 216 (99.5) | 1 (0.5) | 216 (99.5) | 1 | 216 | 0 | 0 | 100 | 100 | 1 [1–1] | 1 |
| HCoV-HKU1 | 1 (0.5) | 216 (99.5) | 1 (0.5) | 216 (99.5) | 1 | 216 | 0 | 0 | 100 | 100 | 1 [1–1] | 1 |
| Influenza A | 2 (0.9) | 215 (99.1) | 2 (0.9) | 215 (99.1) | 2 | 215 | 0 | 0 | 100 | 100 | 1 [1–1] | 1 |
| Influenza B | 13 (7.4) | 204 (92.6) | 6 (2.8) | 211 (97.2) | 5 | 199 | 8 | 1 | 38.5 | 99.5 | 0.51 [0.17–0.76] | 0.03 |
| Influenza A H1N1 2009 | 0 (0) | 217 (100) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| HBoV | 15 (6.9) | 202 (93.1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| EV | 17 (7.8) | 200 (92.2) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| PeV | 2 (0.9) | 215 (99.1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| Mpneu | 0 (0) | 217 (100) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
⁎
P-value for McNemar test. PPA = Positive Percentage Agreement; NPA = Negative Percentage Agreement; RV = Rhinovirus; HCoV = Human Coronavirus (HCoV-229E; HCoV-OC43; HCoV-NL63; HCoV-HKU1); HPIV = Human Parainfluenzavirus (HPIV-1; HPIV-2; HPIV-3; HPIV-4); HMPV = Human Metapneumovirus; HRSV = Human Respiratory Syncytial Virus; HAdV = Human Adenovirus; HBoV = Human Bocavirus; EV = Enterovirus; PeV = Parechovirus; Mpneu = Mycoplasma pneumoniae.