Fig. 7.

Anisomycin specifically decreases −1 ribosomal frameshifting efficiency and has antiviral activity. (a) The effects of anisomycin on programmed ribosomal frameshifting were assayed in intact yeast cells in vivo, and in translationally competent rabbit reticulocyte extracts in vitro. The indicated drug concentrations were subinhibitory for cell growth and division (in vivo), and for overall translation (both in vivo and in vitro) (not shown). Anisomycin specifically inhibits programmed −1 ribosomal frameshifting in both systems. (b) Yeast cells harbouring the L-A and M₁ viruses were cultured in the presence of the indicated concentrations of anisomycin for the indicated times, after which the percentage of cells retaining killer activity was determined.