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In vitro IFN γ production by lymphocytes harvested from mesenteric lymph nodes post challenge then cultured with native leukotoxin. Measured using an IFNγ test kit (limit of detection 80 pg/ml, Bovigam, Pfizer). OD ratio = ratio in culture supernatant to IFN γ positive standard. Incubation time = time in culture prior to sampling.
