Table 1.

Disinfectants and concentrations tested against a tissue culture adapted porcine epidemic diarrhea virus strain in both cell culture media and 10% (v/v) swine feces slurry. Testing was performed in triplicate resulting in 72 samples for each temperature tested. This procedure was replicated for each temperature (37 °C, 4 °C and −20 °C) for a grand total of 216 samples.

Study group	PEDV status to contaminate petri dishes	Treatment with disinfectant (dilution)	Temperatures	Contact time (min)
Negative control	Neg; in culture medium or fecal slurry	Water	37 °C, 4 °C, or −20 °C	60 or 90
Positive control	Pos; in culture medium or fecal slurry	Water	37 °C, 4 °C, or −20 °C	60 or 90
Quaternary ammoniuma	Pos; in culture medium or fecal slurry	1.5:128	37 °C, 4 °C, or −20 °C	60 or 90
Phenolb	Pos; in culture medium or fecal slurry	1:256	37 °C, 4 °C, or −20 °C	60 or 90
Quaternary ammonium/glutaraldehyde combinationc	Pos; in culture medium or fecal slurry	1:256	37 °C, 4 °C, or −20 °C	60 or 90
Oxidizing agentd (0.5%)	Pos; in culture medium or fecal slurry	1:200	37 °C, 4 °C, or −20 °C	60 or 90
Oxidizing agentd (1%)	Pos; in culture medium or fecal slurry	1:100	37 °C, 4 °C, or −20 °C	60 or 90
Oxidizing agentd (2%)	Pos; in culture medium or fecal slurry	1:50	37 °C, 4 °C, or −20 °C	60 or 90
Sodium hypochloritee (0.17%)	Pos; in culture medium or fecal slurry	1:50	37 °C, 4 °C, or −20 °C	60 or 90
Sodium hypochloritee (0.52%)	Pos; in culture medium or fecal slurry	1:16	37 °C, 4 °C, or −20 °C	60 or 90
Sodium hypochloritee (1.03%)	Pos; in culture medium or fecal slurry	1:8	37 °C, 4 °C, or −20 °C	60 or 90
Sodium hypochloritee (2.06%)	Pos; in culture medium or fecal slurry	1:4	37 °C, 4 °C, or −20 °C	60 or 90

^aRoccal-D Plus; Zoetis, Florham Park, New Jersey.

^b1-Stroke Environ; STERIS Corporation, Mentor, Ohio.

^cSynergize; Preserve International, Reno, Nevada.

^dVirkon S; DuPont, Wilmington, Delaware.

^eClorox Regular-Bleach (8.25% sodium hypochlorite); The Clorox Company, Oakland, California.