Table 2.
Number of samples for each treatment group that tested positive for porcine epidemic diarrhea virus (PEDV) with RT-PCR for the disinfectants tested in the presence and absence of a 10% (v/v) fecal slurry at three different temperatures. Each treatment was tested in triplicate.
| 37 °C |
4 °C |
−20 °C |
||||
|---|---|---|---|---|---|---|
| Disinfectant | Cell culture medium | Fecal slurry | Cell culture medium | Fecal slurry | Cell culture medium | Fecal slurry |
| Quaternary ammonium | 3 | 3 | 3 | 3 | 3 | 3 |
| Phenol | 3 | 3 | 3 | 3 | 3 | 3 |
| Quaternary ammonium/glutaraldehyde combination | 3 | 3 | 3 | 3 | 3 | 3 |
| Oxidizing agent (0.5%) | 0 | 3 | 3 | 3 | 3 | 3 |
| Oxidizing agent (1%) | 1 | 3 | 3 | 3 | 3 | 3 |
| Oxidizing agent (2%) | 3 | 3 | 3 | 3 | 3 | 3 |
| Sodium hypochlorite (0.17%) | 3 | 3 | 3 | 3 | 3 | 3 |
| Sodium hypochlorite (0.52%) | 2 | 2 | 3 | 3 | 3 | 3 |
| Sodium hypochlorite (1.03%) | 0 | 0 | 3 | 0 | 1 | 2 |
| Sodium hypochlorite (2.06%) | 0 | 1 | 0 | 0 | 2 | 1 |
| Negative controls | 0 | 0 | 0 | 0 | 0 | 0 |
| Positive controlsa | 3 | 3 | 3 | 3 | 3 | 3 |
a
Viable PEDV was recovered from the positive control samples but PEDV could not be recovered from any of the disinfectant treated or negative control samples when tested with virus isolation.