Table 2.

Expression of recombinant Lf by various transgenic organisms.

Expression system	Lf origen	Expression level	Year/reference
Bacteria
E. coli	bLfc	10 mg/L	2007 [201]
	Lfc	60 mg/L	2006 [202]
	bLfc	2 mg/L	2006 [203]
	Kumin Lf	17 mg/L	2010 [204]
Lactobacillus casei	hLf	10.6 mg/mL	2010 [205]
Rhodococcus erythropolis	bLf C-lobe	3.6 mg/mL	2006 [206]
Yeast
Pichia pastoris	hLf	115 mg/L	2004 [207]
	cLf	2 mg/L	2007 [208]
	pLf	12 mg/L	2002 [209]
	Yak Lf	40 mg/L	2006 [210]
	eLf	40 mg/L	2002 [211]
	hLf	1200 mg/L	2008 [212]
Pichia methanolica	pLf	NR	2007 [213]
	bLfc	90 mg/L	2007 [214]
Fungi
Aspergillus awamori	hLf	2 g/L	1995 [215]
Aspergillus oryzae	hLf	25 mg/L	1992 [216]
Insects
Spodoptera frugiperda	hLf	9.5 mg/L	1998 [217]
Bombyx mori	hLf	65 mg/L	2005 [218]
	hLf	13.5 μg/1–2 × 105 cells	2006 [219]
	pLf	205 μg/pupa	2005 [220]
Cell lines
Baby hamster kidney (BHK)	hLf	20 mg/L	1991 [221]
Cell culture (human embryo kidney)	hLf	16 mg/L	2009 [222]
Mammals
Goat	hLf	0.756 mg/L	2008 [223]
	hLf	2 g/L	2007 [224]
Mice	hLf	2.5 mg/mL	1997 [225]
	hLf	2.5 mg–200 μg/mL milk	1997 [226]
Rabbit	hLf	2.3 mg/mL milk	2008 [227]
Bovine	hLf	1 g/L milk	2002 [228]
	hLf	2.9 mg/mL	2006 [229]
Plants
Nicotiana benthamiana	hLf N-lobe	0.6% soluble protein	2004 [230]
Nicotiana tabacum	hLf	NR	1994 [231]
	hLf	0.1–0.8% soluble protein	1998 [232]
	hLf	4.3% soluble protein	2003 [233]
	bLf	NR	2011 [234]
Rice	hLf	1.6 mg/g seed	2004 [235]
	hLf	0.5% total biomass	2005 [236]
	hLf	2–4% soluble protein	2003 [237]
	hLf	0.1% soluble protein	2010 [238]
Potato	hLf	0.1% soluble protein	2000 [239]
Sweet potato (Ipomoea batata)	hLf	3.2 μg/mg total protein	2006 [240]
Panax ginseng	hLf	3.0% soluble protein	2003 [241]
Tomato (Lycopersicon esculentum)	hLf	NR	2002 [242]
Maize	hLf	NR	2001 [243]
Alfalfa (Medicago sativa)	hLf	NR	2005 [244]
Barley	hLf	NR	2011 [245]

(h = human, b = bovine, c = caprine, p = porcine, e = equine, Lfc = lactoferricin, NR = not reported).