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Comparison of effective annealing temperature between conventional primer and DPO primer (A) and specific PCR product of target gene of each virus (B). M: DNA Marker; numbers 1–8: Annealing temperature at 40 °C, 43.2 °C, 48.2 °C, 51 °C, 54 °C, 56.8 °C, 61.8 °C, and 65 °C, respectively; NC: negative control.
