. 2019 Aug 12;47:101435. doi: 10.1016/j.mcp.2019.101435

Table 1.

Primers used in this study.

Viruses	Primer ID	Sequences (5′–3′)	Reference	Product size
PEDV	PEDV-N-F^a	ATGGCTTCTGTCAGCTTTCA	GenBank DQ355221.1	1326 bp
	PEDV-N-R^a	TTAATTTCCTGTATCGAAGATCTCG	GenBank DQ355221.1
	PEDV-DPO-F^b	GGTATTGGAGAAAATCCTGACAGGIIIII^dGCAACAGCA		251 bp
	PEDV-DPO-R^b	GACGCATCAACACCTTTTTCGIIIIITTCCGCATC
	PEDV-C-F^c	GGTATTGGAGAAAATCCTGACAGGCATAAGCAACAGCA		251 bp
	PEDV-C-R^c	GACGCATCAACACCTTTTTCGACAAATTCCGCATC
TGEV	TGEV-N-F^a	ATGGCCAACCAGGGACAA	GenBank KU729220.1	1149 bp
	TGEV-N-R^a	TTAGTTCGTTACCTCATCAATTATC	GenBank KU729220.1
	TGEV-DPO-F^b	CTGTTCTTGCCGCACTTAAAAIIIIIGGTGTTGAC		180 bp
	TGEV-DPO-R^b	TAGCTCCATAAAATCTTGTCACATCIIIIITACCTGCAG
	TGEV-C-F^c	CTGTTCTTGCCGCACTTAAAAAGTTAGGTGTTGAC		180 bp
	TGEV-C-R^c	TAGCTCCATAAAATCTTGTCACATCACCTTTACCTGCA
PoRV	PoRV-VP7-F^a	ATGTATGGTATTGAATATACCACAG	GenBank JN388691.1	981 bp
	PoRV-VP7-R^a	CTAGACTCGGTAATAAAAGGCAG	GenBank JN388691.1
	PoRV-DPO-F^b	TGTTTTTAACAAAAGGATIIIIIACAGGGTCA		318 bp
	PoRV-DPO-R^b	CTGTAGTCGAACATCCTAIIIIIAGAGTCTGC
	PoRV-C-F^c	TGTTTTTAACAAAAGGATGGCCAACAGGGTCA		318 bp
	PoRV-C-R^c	CTGTAGTCGAACATCCTATCCCGAGAGTCTGC
PDCoV	PDCoV-N-F^a	ATGGCCGCACCAGTAGTCCCTA	GenBank MH715491.1	1029 bp
	PDCoV-N-R^a	CTACGCTGCTGATTCCTGCTTT	GenBank MH715491.1
	PDCoV-DPO-F^b	AGGCTACTCATCCTCAGTTTCGTGGIIIIIGAGTTCCGC		400 bp
	PDCoV-DPO-R^b	GGGCCACTTACACGCTCCTGAGGTCIIIIICTAGCGTTG
	PDCoV-C-F^c	AGGCTACTCATCCTCAGTTTCGTGGCAATGGAGTTCCGC		400 bp
	PDCoV-C-R^c	GGGCCACTTACACGCTCCTGAGGTCTTCCTCTAGCGTTG

Primers were used to amplify the full-length of the target gene for positive standard control.

DPO primers were used to establish detection method.

Conventional primers were used as control for comparison with DPO primers.

“I” means deoxyinosine.