Table 2.

Repeatability of DPO-based real-time RT-PCR assay.

Standards	Concentration (copies/μL)	n	Intra-assay variability		Inter-assay variability
			X±SD (Ct)	CV (%)	X±SD (Ct)	CV (%)
pMD-TGEV-N	1.52 × 107	5	13.28 ± 0.08	0.60	12.83 ± 0.12	0.83
	1.52 × 105	5	21.43 ± 0.13	0.22	21.19 ± 0.13	0.52
	1.52 × 103	5	26.84 ± 0.09	0.36	28.19 ± 0.19	0.67
pMD-PEDV-N	1.64 × 107	5	14.13 ± 0.09	0.63	13.73 ± 0.15	0.83
	1.64 × 105	5	19.51 ± 0.11	0.56	20.26 ± 0.14	0.69
	1.64 × 103	5	26.16 ± 0.08	0.31	27.54 ± 0.16	0.58
pMD-PoRV-VP7	2.27 × 107	5	14.97 ± 0.07	0.46	13.51 ± 0.22	0.72
	2.27 × 105	5	19.23 ± 0.12	0.62	20.79 ± 0.29	0.89
	2.27 × 103	5	25.81 ± 0.10	0.38	28.11 ± 0.17	0.60
pMD-PDCoV-N	1.58 × 107	5	15.38 ± 0.19	0.93	14.48 ± 0.12	0.83
	1.58 × 105	5	19.96 ± 0.23	0.85	20.76 ± 0.25	0.97
	1.58 × 103	5	26.01 ± 0.16	0.62	26.71 ± 0.28	0.95