Table 3.
Detection results of clinical samples.
| Year | n | Conventional RT-PCR assay |
Prevalence rate TGEV/PEDV/PoRV/PDCoV | DPO-based real-time RT-PCR assay |
Prevalence rate TGEV/PEDV/PoRV/PDCoV |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TGEV +/− |
PEDV +/− |
PoRV +/− |
PDCoV +/− |
TGEV +/− |
PEDV +/− |
PoRV +/− |
PDCoV +/− |
||||
| 2017 | 376 | 25/351 | 64/312 | 15/361 | 10/366 | 6.65%/17.02%/3.99%/2.66% | 29/347 | 71/305 | 17/359 | 11/365 | 7.71%/18.88%/4.52%/2.93% |
| 2018 | 296 | 5/291 | 54/242 | 11/285 | 14/282 | 1.69%/18.24%/3.72%/4.73% | 6/290 | 57/239 | 12/284 | 15/281 | 2.03%/19.26%/4.05%/5.07% |
| Total | 672 | 30/642 | 118/554 | 26/646 | 24/648 | 4.46%/17.56%/3.87%/3.57% | 35/637 | 128/544 | 29/643 | 26/646 | 5.21%/19.05%/4.32%/3.87% |
| Coincidence ratesa between conventional RT-PCR assay and DPO-based real-time RT-PCR assay | TGEV 85.71% | PEDV 92.19% | PoRV 89.66% | PDCoV 92.31% | |||||||
a
Coincidence rate was the positive results detected by conventional RT-PCR/the positive results detected by DPO-based real-time RT-PCR.